Coast Guard officials' estimation of when the missing Titanic submersible would run out of oxygen has passed.

The missing OceanGate submersible, which left for its expedition to view the Titanic wreckage on Sunday morning, disappeared roughly one hour and 45 minutes after its dive began. The vessel is intact with 96 hours of oxygen, but officials said the air could run out as early as 7:08 a.m Eastern time on Thursday.

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The Titan submersible is a small submarine designed by OceanGate to carry five passengers, including one pilot and four crew members, to explore the wreckage of the Titanic passenger ship.

While the Titan has been touted by OceanGate as a state-of-the-art machine, experts and observers expressed concerns about several technical features. In a letter sent by the Marine Technology Society, the group expressed its "unanimous concern" regarding OceanGate's decision to forgo DNV-GL class rules.

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The remains of the Titanic are located in the Atlantic Ocean, roughly 370 miles south of Newfoundland, Canada, and the shipwreck is about 2.4 miles underneath the surface of the water.

The five people on board the vessel include Hamish Harding, the chairman of Action Aviation; Shahzada Dawood, the vice chairman of Engro Corporation Limited; Dawood's son Suleman; Paul-Henri Nargeolet, a diver and Titanic researcher and director of underwater research at RMS Titanic; and OceanGate CEO Stockton Rush.