The heads of three universities will appear before Congress on Tuesday, becoming the latest batch of leaders who Republicans have called to Washington over allegations of campus antisemitism.

In a hearing before the House Committee on Education and the Workforce, leaders from the City University of New York, Georgetown University and the University of California, Berkeley, will testify about “the role of faculty, funding and ideology” in antisemitism.

The first hearings targeted Ivy League universities, but Republicans have widened their lens to other kinds of institutions, which they say also failed to keep Jewish students safe when pro-Palestinian protests swept campuses around the country.

While the protests have died down substantially since last year, Tim Walberg, a Michigan Republican and the committee’s chair, said in a statement that lawmakers “continue to see antisemitic hatred festering at schools across the country.”

Mr. Walberg echoed President Trump, who campaigned on punishing universities that he said had not done enough to curb antisemitism. The Trump administration has taken away major sums of money — billions in Harvard’s case — from top universities. A federal task force on antisemitism has singled out many institutions for investigation, and federal agents have detained international students who were involved in pro-Palestinian activism.

The Republican-led hearings began before the second Trump administration, months after Hamas attacked Israel in October 2023, which led to Israel’s ongoing invasion of Gaza. The presidents of M.I.T., Harvard University and the University of Pennsylvania were called in for the first hearing, which turned into a disastrous spectacle for the leaders.