More than two years ago, the actress Riley Keough settled a high-profile legal dispute with her grandmother Priscilla Presley over control of the Presley family trust. The litigation arose after the sudden death of Lisa Marie Presley in January 2023, placing the Presley family’s messy affairs into public view.

Behind the scenes, yet another acrimonious fight over money was brewing. Priscilla Presley had brought on a Florida auctioneer and a Tennessee entrepreneur to help manage her business affairs in 2022, a time when, the former advisers say in court documents, she had spent her way into financial straits.

By late 2023, however, the relationship had soured and the parties began trading lawsuits. In court papers filed in Florida and California, the advisers who managed Ms. Presley’s business interests under the name Priscilla Presley Partners have accused her of breaching their contracts, while Ms. Presley has accused her former partners of financial elder abuse and other offenses.

The latest salvo arrived this week at Los Angeles Superior Court, where the auctioneer, Brigitte Kruse, and the entrepreneur, Kevin Fialko, sued Ms. Presley again, accusing her of fraud in court papers that seek at least $50 million in damages.

In the court papers, lawyers for Ms. Kruse and Mr. Fialko also made a bold accusation that is sure to escalate the dispute: After Lisa Marie was rushed to the hospital in 2023 following a cardiac arrest, the lawsuit says, “Priscilla pulled the plug” on her daughter as part of an effort to “regain control” of the family trust.

“Priscilla, who knew that Lisa was in the process of taking steps to remove her as the sole trustee of Lisa’s irrevocable life insurance trust, saw an opportunity to regain control,” the lawsuit said.

Priscilla Presley had acted, the lawsuit said, even though Lisa Marie had an advance health care directive in place that made clear that she wanted her life “to be prolonged as long as possible.”

A lawyer for Ms. Presley, Marty Singer, condemned the allegation in a two-page statement in which he called the new filing “one of the most shameful, ridiculous, salacious and meritless lawsuits I have seen in my practice.”

“This is nothing more than a sad and vicious attempt to falsely tarnish the reputation of an 80-year-old woman in blatant retaliation for bringing a lawsuit to redress the wrongful conduct of Brigitte Kruse, Kevin Fialko and their co-conspirators,” the statement said.

The new lawsuit does not detail how hastening Lisa Marie’s death in 2023 would have affected matters involving control of the trust. The New York Times has previously reported that as early as 2016, Lisa Marie had authorized an amendment to the family trust that removed Priscilla as a trustee. But it is also not clear the extent to which Priscilla Presley knew in 2023 of the existence of the documents that said she had already been removed.

A lawyer for the plaintiffs did not respond to a request for comment.

In his statement, Mr. Singer labeled the lawsuit “a disgusting publicity stunt,” adding that “accusing a grieving mother of contributing to her daughter’s death is not savvy advocacy; it is malicious character assassination.”

Specifically, the lawsuit filed by Ms. Kruse and Mr. Fialko said Ms. Presley had “pulled the plug within hours of Lisa being admitted” to the hospital “and before her granddaughter, Riley, was able to get to the hospital.”

A lawyer and representatives for Ms. Keough, the trustee of the Presley family trust, did not respond to requests for comment.

In his statement, Mr. Singer said Ms. Keough “stands behind her grandmother 100 percent and is equally disgusted with this latest, vicious attempt by Kruse to attempt to ruin her grandmother’s life.”

While the Elvis brand takes in more than $100 million a year, business dealings over the years have reduced the Presley family stake in Elvis Presley Enterprises, a venture operated by Authentic Brands Group, to a 15 percent share. Profits from that stake are funneled to the family’s Promenade Trust. Elvis Presley Enterprises operates Elvis’s former home, Graceland, in Memphis, Tenn. But the trust retains the house itself.

In 2023, when Priscilla Presley disputed the authenticity of documents that removed her as a Promenade trustee, Ms. Keough and Ms. Presley ultimately settled, though the financial details were not disclosed at the time it was announced. The lawsuit filed this week by Ms. Presley ‘s former business partners said Ms. Kruse and Mr. Fialko “ultimately brokered a deal, where Priscilla was to be paid $2.4 million in aggregate, and they also brokered a seven-figure deal for Navarone Garibaldi, Priscilla’s son by her longtime partner, Marco Garibaldi, who otherwise would have been entitled to nothing.”

In their lawsuit, Ms. Kruse and Mr. Fialko say that they “invested thousands of hours of their time" and “millions of dollars into Priscilla,” building her “name, image and likeness” to enhance Ms. Presley’s brand. They say in court papers that Ms. Presley licensed her N.I.L. rights to their Priscilla Presley Partners and other related companies to pursue various business objectives. Ms. Presley entered into multiple operating agreements with those companies, the lawsuit said.

But before entering those agreements, Ms. Presley never disclosed to Ms. Kruse and Mr. Fialko “that she previously sold the rights to exploit her name, image and likeness years before, in 2005” to Authentic Brands Group for roughly $6.5 million, the court papers say.

Then, the lawsuit says, just days before the $2.4 million settlement with Ms. Keough was finalized, Ms. Presley cut off Ms. Kruse and Mr. Fialko. The plaintiffs say they were never compensated for their work reviving Ms. Presley’s finances or resolving her many legal disputes.

Ms. Kruse and Mr. Fialko also complained in the lawsuit that they were disinvited from the premiere of Sofia Coppola’s biopic “Priscilla,” at the Venice Film Festival, despite having brokered “additional compensation for Priscilla from A24,” according to the lawsuit. Excluding her business partners from the premiere was a breach of contract, the lawsuit says, essentially because doing so prevented the partners from advancing their businesses.

They say in court papers that Ms. Presley is continuing to promote herself in a way that violates the terms of their agreement.

A representative for A24 declined to comment.

In his statement, Mr. Singer restated elements of the lawsuit Ms. Presley filed more than a year ago in which she said she had been the victim of “a concerted effort” by Ms. Kruse and Mr. Fialko “to isolate her from her trusted advisors and dupe her into signing a series of agreements.”

Specifically, the lawsuit says Ms. Presley was pressured into signing more than 20 different agreements she had never seen before in a 27-minute session at Ms. Kruse’s house. Ms. Presley also accused Ms. Kruse in court papers of demanding that she give up her cellphone so that all communication could be overseen by Ms. Kruse.

In their own court filings, lawyers for the former advisers have denied various aspects of the claims and asked a judge to strike the request for putative damages.

A case management conference in connection with the latest lawsuit has been set for February 2026.

Susan C. Beachy contributed research.