Among the long list of political adversaries President Trump has targeted in his second term are several Black women in powerful positions.

There’s Lisa Cook, who serves on the Federal Reserve’s Board of Governors, which has stood in the way of Mr. Trump’s desire to lower interest rates. And Fani T. Willis, the Georgia prosecutor who brought election interference charges against him.

And now, there is Attorney General Letitia James of New York, who brought a civil case against Mr. Trump and won.

All three women have risen to their posts as firsts. Ms. James is the first woman in New York to be elected attorney general and the first African American woman elected to statewide office. Ms. Willis is the first woman to serve as the district attorney for Fulton County, while Ms. Cook is the first Black woman to serve on the Federal Reserve board.

The president has denigrated each of them and tried to destroy their ability to work against his interests.

And Mr. Trump’s singling out of Black women who have crossed him comes as he has fired a series of Black officials from high-profile positions in what is now an overwhelmingly white administration that has banished diversity, equity and inclusion programs across the federal government.

On Thursday, Ms. James was indicted on bank fraud and false statement charges in Virginia in a case led by a federal prosecutor handpicked by Mr. Trump. The indictment came months after the president escalated a pressure campaign against Ms. James, as the Justice Department opened a civil rights investigation into her office and appointed a special prosecutor to investigate her real estate holdings.

In August, Ms. Cook was accused of mortgage fraud by Bill Pulte, the director of the Federal Housing Finance Agency, who has made similar claims about Ms. James. A week later, Mr. Trump said in a social media post that he was firing Ms. Cook. (She has denied the allegations and sued Mr. Trump, and the Supreme Court last week allowed Ms. Cook to stay on the board for now.)

Ms. Willis is also being investigated by the Justice Department. Prosecutors from the U.S. attorney’s office for the Northern District of Georgia have issued a subpoena for her travel records.

Mr. Trump has attacked Ms. Willis and Ms. James verbally. He has called Ms. Willis racist and said she was out to “get Trump.” The president has called Ms. James “scum” and other insulting names, including a nickname for her that is reminiscent of a racial slur.

When Ms. James ran for attorney general in 2018, she said she was motivated in large part by a desire to hold Mr. Trump accountable. After she won, Mr. Trump called her “yet another” attorney general who “does little else but rant, rave & politic against me” in a social media post.

Ms. James sued Mr. Trump in 2022, accusing him of defrauding lenders by overvaluing his assets by more than $2 billion. She won, and in February 2024, a New York State judge levied a fine that with interest grew to more than half a billion dollars. That penalty was thrown out by an appeals court in August, and the case is set to be reviewed by New York’s highest court.

In a statement on Thursday, Ms. James called the charges against her “baseless.” She said she stood by her office’s case against Mr. Trump, his family and his business interests.

“We conducted a two-year investigation based on the facts and evidence — not politics,” Ms. James said.

Grace Ashford contributed reporting.