The U.S. attorney in Albany has issued two broad subpoenas to Attorney General Letitia James of New York, one related to her office’s civil fraud case against President Trump, according to three people with knowledge of the matter.

The second subpoena is related to another of the office’s long-running cases, against the National Rifle Association, the people said.

Ms. James has been one of Trump’s fiercest adversaries since she first ran for attorney general in 2018.

In 2022, she sued him, accusing him of fraudulently overvaluing his assets by billions of dollars. Mr. Trump lost and was penalized with a fine that, with interest, now runs more than half a billion dollars. The case is on appeal.

Ms. James’s personal lawyer, Abbe Lowell, called any investigation into Mr. Trump’s fraud case “the most blatant and desperate example of this administration carrying out the president’s political retribution campaign.”

“Weaponizing the Department of Justice to try to punish an elected official for doing her job is an attack on the rule of law and a dangerous escalation by this administration,” he said. “If prosecutors carry out this improper tactic and are genuinely interested in the truth, we are ready and waiting with facts and the law.”

Geoff Burgan, a spokesman for Ms. James, said: “We stand strongly behind our successful litigation against the Trump Organization and the National Rifle Association, and we will continue to stand up for New Yorkers’ rights.”

A Justice Department spokesman declined to comment. The U.S. attorney’s office did not immediately respond to a request for comment.

The subpoenas appear unrelated to a case involving Ms. James’s personal real-estate transactions. The existence of that case was publicly confirmed earlier this year by the F.B.I. director, Kash Patel, but it is unclear whether or how it has progressed.

This is a developing story and will be updated.