Harvey Weinstein, the disgraced Hollywood producer, pleaded not guilty to a new sex-crime indictment in a Manhattan court on Wednesday, months after a previous conviction was thrown out.

The indictment accuses Mr. Weinstein of sexually assaulting a woman in a Manhattan hotel in 2006.

The Manhattan district attorney’s office, which is prosecuting Mr. Weinstein on the charge of committing a criminal sexual act in the first degree, is also retrying the earlier case, in which the verdict was overturned by the New York Court of Appeals.

Mr. Weinstein, 72, has also been convicted in California on sex charges and faces a prison term there.

Prosecutors said in a hearing last week that Mr. Weinstein’s new indictment would remain sealed until he was well enough to be in court. That day came Wednesday.

Mr. Weinstein, who on Sept. 8 had emergency heart surgery, was brought into the courtroom in a wheelchair, wearing dark rimmed glasses and a dark suit, displaying a bandage on his right hand and holding two books. As he was arraigned, Mr. Weinstein sat next to his lawyers, only replying “not guilty.”

In 2020, Mr. Weinstein was convicted in New York of raping an aspiring actress in a hotel room seven years earlier. He was also convicted of assaulting a former television production assistant, who testified that he had forced oral sex on her in his Manhattan apartment in July 2006.

But New York’s highest court found this year that the judge who presided over the trial had erred by allowing prosecutors to call several accusers as witnesses, even though their allegations had not led to charges.

Immediately after the court’s April decision, the Manhattan district attorney, Alvin L. Bragg, said that he would prosecute Mr. Weinstein again.

This is a developing story and will be updated.