City investigators seized cash on Thursday from safes at the New York City Sheriff’s Office, which has raided hundreds of illegal cannabis shops and confiscated millions of dollars in proceeds and merchandise, according to a spokeswoman for Mayor Eric Adams’s administration.

The Department of Investigation is looking into whether the office improperly took money from stores that it had closed and padlocked, according to three people with knowledge of the matter, who requested anonymity to discuss the case without authorization.

The spokeswoman, Liz Garcia, said the agents had been called to the agency’s Queens headquarters at the request of Sheriff Anthony Miranda, who was appointed by Mr. Adams. She said that on Wednesday, Mr. Miranda had discovered money that apparently was not properly accounted for.

“Sheriff Miranda learned of seemingly unvouchered cash held in safe boxes and self-reported the incident to his supervisors,” Ms. Garcia said in a statement Thursday evening. “His supervisors then reported this to the Department of Investigation. We expect every city employee to follow proper procedures.”

The Department of Investigation also has also been looking into whether Mr. Miranda, a former police sergeant, or others acting on his behalf solicited money for a police fraternal organization from merchants in exchange for protection against raids, said two of the people, who are law enforcement officers.

“There’s no factual basis to any of that nonsense,” Mr. Miranda said when reached by telephone Thursday night.