Police have charged a suspect with shooting rookie NYPD cop Brett Boller in Queens, following his arrest by federal officers in the Bronx overnight.

Devin Spraggins, 22, is expected to face charges of first-degree attempted murder of a police officer, second-degree criminal possession of a weapon and obstruction of government administration, New York City Police Commissioner Keechant Sewell said Friday.

Spraggins allegedly fired a single shot at Officer Boller after a violent dispute over a seat onboard an MTA bus in Jamaica Wednesday.

Spraggins was nabbed around 8 p.m. Thursday in the Wakefield section of the Bronx by members of the US Marshals Fugitive Task Force.

A 9mm handgun was recovered from the scene. It’s unclear whether it was the same weapon used in the cop’s shooting, but shells and a magazine of the caliber were found at the site of the schooting.

Spraggins reportedly has no prior criminal history in New York.

The shooting incident began at about 3:20 p.m. Wednesday, when Spraggins pulled a gun on a bus passenger during an argument over a seat, prompting the MTA driver to flag down two police officers who were in the area, cops said.

Spraggins then fled on foot after pushing an NYPD officer out of the way. But Boller — who has been on the force only three month — gave chase.

Harrowing surveillance video obtained by The Post showed Spraggins tussling with Boller on the ground in the middle of 161st Street, before he allegedly open fire, striking the officer in the right hip.

As the gunman ran away, he ditched his jacket, mask and sweatshirt, which were later recovered from a parking garage.

Boller was helped by his fellow NYPD officers at the scene, before being rushed to Jamaica Hospital, where he was recovering following surgery.

Boller, from Hauppauge, Long Island, is the son of an NYPD inspector in the Brooklyn North precinct.

Police were offering a combined reward of $20,000 for information leading to the shooter’s arrest.