The company whose bus crashed on a Long Island school trip last week, killing two teachers and leaving five students in critical condition, has been cited for federal safety violations more than two dozen times — and was on a recent list of “unacceptable operators.”

Regency Transportation LTD has had 42 inspections of its buses over the last two years, before one careened off the I-84 on Thursday, injuring at least 40 Farmingdale High School students and faculty on their way to a band camp in Pennsylvania, online records show.

It has been cited for 25 violations since 2021 — failing five inspections in the 2023 fiscal year, according to records from the Federal Motor Carrier Safety Administration.

Among the violations were “defective bus emergency exits,” “inoperable brake lamps” and “inoperable headlamps,” as well as oil and grease leaks, the agency records show.

Regency was placed on the state’s most recent list of “unacceptable operators” because of the failed inspections, according to lohud.com.

A company bus was also involved in a two-vehicle crash on Long Island last year that resulted in one injury — although no citations were issued, the outlet noted.

However, it still had “valid operating authority from the State Department of Transportation and a valid semi-annual inspection,” officials stated, noting that the unidentified driver was also “properly licensed.”

The bus operator, Nasconset-based Regency Buses, has eight vehicles and employs 14 drivers, the outlet said. The company did not respond to a request for comment from The Post on Monday.

Thursday’s crash came as a six-bus convoy from Farmingdale High School was taking the school marching band and several adults to a camp in Greeley, Pennsylvania.

One of the buses veered off the highway and down a 50-foot ravine shortly before 1:20 p.m.

The crash killed marching band director Gina Pellettiere, 43, and retired music teacher Beatrice Ferrari, 77, who was on the trip as a chaperone. Five students were critically injured.

The fatal crash is now being investigated by the National Transportation Safety Board.

“Our goal is to find out what happened, why it happened and to make sure that something like this never happens again,” John Humm, who is leading the investigation for the agency, told lohud.

A preliminary New York State Police probe determined that a failure of the front tire may have played a role in the crash, but Humm said it’s “really premature” at this stage to say that was the cause.