One man was shot, a second man stabbed and a third pepper sprayed on the subway in separate acts of violence in the city overnight, police said.

In the latest incident, a man was pepper-sprayed aboard an E train at Broadway and 34th Street shortly before 5:30 a.m. Saturday, then robbed of his jacket containing his cell phone, cops said.

The victim, whose age was not immediately available, was taken by EMS to Lenox Hill Hospital in stable condition, police said. Police described the suspect as a man in his 50s, with a slim build wearing a white jacket.

There are no arrests.

Hours earlier, around 2:45 a.m., a 33-year-old man walked into Harlem Hospital with a gunshot wound in the abdomen, cops said.

The victim, who was brought to the hospital by private means, said he had been shot earlier on the 3700 block of 10th Avenue, Inwood, police said.

He’s listed in stable condition.

Shortly before 1:30 a.m., a 19-year-old man was stabbed in the back on McDonald and Ditmas Avenues in Brooklyn, police said.

EMS took the victim to Maimonides Medical Center in stable condition, cops said.

Marco Bautista, 25, of the borough’s Kensington section, was arrested and charged with assault and criminal possession of a weapon, the NYPD said.

It’s unclear what sparked the bloodshed.