A physician’s assistant allegedly enlisted a patient to kill her own brother in a depraved murder-for-hire plot, The Post has learned.

LaDonna Card, 58, who worked at Mount Sinai/Beth Israel Medical Center for 33 years, allegedly told the patient at the 125th Street methadone clinic in Harlem that she wanted him to kill her older sibling on multiple occasions, according to authorities and the patient.

“Cut his f–king arm or leg off and let him bleed the f–k out,” Card allegedly said.

The patient, recovering opioid addict Michael Gessow, said he secretly recorded Card while pretending to be on board with the plot. He said he then alerted his drug counselor.

Card was arrested in January for criminal solicitation, the NYPD confirmed.

She was arraigned in Manhattan Criminal Court, where Manhattan District Attorney Alvin Bragg’s office consented to Card being released on her own recognizance because “the charge was not bail eligible,” said Bragg spokeswoman M’Niyah Lynn.

The alleged target, Kermit Card, 61, a retired city Correction officer, admitted he lives in fear of his scheming sister.

“I bought a bulletproof vest,” he told The Post before declining further comment.

“She didn’t care. She just wanted him gone,” Gessow told The Post during an exclusive sitdown at his Bronx home.

Gessow played the shocking 37-minute recording for The Post.

LaDonna Card can be heard making talk and then matter-of-factly advises Gessow he can just “maim” Kermit.

“Like the frog,” she notes of her brother’s first name, referring to the “Muppets” character.

The first conversation took place Nov. 9, when Card said she was having problems with her brother and wished he was gone, according to the criminal complaint.

A second conversation occurred Dec. 8, when Card provided the “informant” with a name, description, and address of her brother, the court papers say.

“I don’t have much money. I want you to make it look like an accident,” Card said, according to the court documents.

“Why she picked me beats the hell out of me,” Gessow, 47, said. “She said something happened with her and her brother because her mother just passed away and the brother took all the money.”

The contract killing would have earned Gessow roughly $10,000, he said.

Gessow, said he never intended to carry out the twisted murder-for-hire plot. Instead he asked his drug counselor, Robert Kirby, how to use his smartphone to audio record. Gessow then recorded the physician assistant during the Dec. 8 conversation and played Kirby the incriminating tape the next day.

Kirby reported the crime to his higher-ups — and was soon fired.

In a June 20 wrongful termination lawsuit filed in Westchester Supreme Court against Mount Sinai Health System and Beth Israel Medical Center, Kirby claimed his bosses canned him because they believed he colluded to record Card.

“He reported a serious crime to the organization and instead of praising him for his heroic work they terminated him. We brought this suit to hold the defendants accountable,” said Kirby’s attorney, Christopher Berlingieri.

Gessow said he’s told his story to the cops and the Manhattan District Attorney’s office, and given the DA a copy of the recording.

“I wasn’t going to say anything at first because, you know, I go to a methadone program, but I’ve been clean for 14 years,” he said.

The hospital declined comment. LaDonna Card could not be reached for comment. Her attorney, Jeffrey Chabrowe did not return messages.

As Card’s case moves through the legal system, Gessow said he’s lost 20 pounds and can’t sleep.

He’s far from happy that she’s walking the streets.

“I’m scared sh-tless right now. It ain’t a joke,” he said.