TAMPA — For a second straight March, the Yankees are holding their breath about Gerrit Cole’s elbow.

The Yankees ace is set to undergo an MRI on his right elbow, a source confirmed to The Post’s Jon Heyman on Friday night, after experiencing some discomfort.

MLB Network first reported the news.

Cole had made his second start of the spring on Thursday afternoon, giving up six runs in 2 ⅔ innings against the Twins while building his pitch count up to 54.

He said after the outing he was pleased with his fastball velocity — which averaged 95.7 mph and topped out at 97.6 mph — but struggled with his command.

Last March, Cole was diagnosed with elbow nerve inflammation and edema after making just one start, which sidelined him until June.

The Yankees already suffered one injury to their rotation this spring as reigning AL Rookie of the Year Luis Gil was diagnosed this week with a high-grade lat strain, which will keep him out at least until June.

— Additional reporting by Jon Heyman