Two brothers were tossed in jail Friday for a wild road-rage clash with an off-duty cop that ended with the officer getting shot in the leg.

In shocking video footage, Shawn, 27, and Edwin Rivera, 32, are seen trying to wrestle a gun away from off-duty cop Christoper Campos in the tense brawl that left him with a bullet to the knee — and the elder Rivera injured by a slug that ripped through his hand, according to a criminal complaint.

The brothers appeared Friday morning in Queens Criminal Court, where Judge Anthony Battisti ordered them held without bail.

The melee began around 12:50 p.m. Wednesday at the intersection of Queens Boulevard and 70th Street in Elmhurst — where Campos, driving a green Kia Soul, tried to get around the brothers’ white Chrysler Pacifica blocking the road.

After Campos got around the car, it sped up — almost hitting the cop’s vehicle, the complaint alleges.

Campos stopped and reversed his car in the intersection toward the Pacifica to see what had happened. He saw Shawn Rivera run toward his car and punch the car’s back window, shattering the glass, authorities charged.

Campos then got out of his car — gun drawn — and told the brothers he was a cop and was going to arrest them, the court papers say.

The incident was filmed with a cellphone by a bystander and caught Edwin saying at one point, “Leave him alone, he’s a cop.” The video also captured Shawn admitting to smashing Campos’ window, the complaint says.

“Get his gun, he’s nothing without his gun,” one of the brothers said, according to the complaint, citing the video.

The siblings pushed Campos — who called for backup — before Edwin put his arms around the cop’s neck and squeezed, causing the officer to fall to the ground, the criminal complaint says.

The video shows Shawn then “join [Edwin] in wrestling” Campos, the complaint alleges.

Edwin tried to get the gun away from Campos by choking him and causing him “to see black and white, have difficulty breathing and begin to lose consciousness,” the complaint alleges.

During the struggle for the weapon, Campos “fired one round of ammunition from his firearm, which struck [Campos] in his leg and which struck” Edwin “in his hand,” the complaint claims.

Campos “is in severe pain and is unable to walk,” and “underwent surgery to his knee,” the complaint says.

The duo are charged with first-degree assault, assault on a police officer, attempted criminal possession of a weapon, strangulation and related crimes. They face up to 25 years in prison if convicted.

“We will not allow our streets to devolve into the Wild West,” Queens District Attorney Melinda Katz said in a statement. “The rule of law and the officers who enforce it must be respected.”

“After clearly identifying himself as a police officer, this off-duty cop was attacked while trying to make an arrest and the charges reflect the seriousness of the assault against him.”

The Riveras’ criminal defense attorney didn’t immediately return a request for comment Friday.