Four people were killed and two others left clinging to life when a massive fire ripped through a Lower Manhattan e-bike store early Tuesday, officials said. 

Two men and two women died in the blaze that erupted around 12:15 a.m. on the first floor of six-story 80 Madison Street, which houses the HQ E-Bike Repair, authorities said. 

Two other women are listed in critical condition, according to police. 

The FDNY reported a total of nine victims in the early-morning inferno, including one firefighter and one EMT, both with minor injuries, cops said. 

Those injured were taken to Bellevue Hospital, Weill Cornell Medical Center and NewYorkPresbyterian facilities, according to the FDNY. 

“We arrived in just about four minutes,” Deputy Assistant Chief John Sarrocco said at the scene. “We found heavy fire in an e-bike store, which is located on the first floor.”

“Unfortunately we had a heavy smoke condition throughout the building.”

The blaze reached three alarms, with 33 units, including 138 fire and EMS personnel, responding to the scene.

It was placed under control around 2:30 a.m. 

The cause remains under investigation.