Applegate's 12-year-old daughter, Sadie Grace, accompanied her in a coordinated black outfit.
Applegate revealed that she was diagnosed with multiple sclerosis (MS) in 2021 while working on the last season of "Dead to Me."
CHRISTINA APPLEGATE REVEALS HOW SHE COPES WITH MS: ‘MY HUMOR SHIELD KEEPS ME OK’
The star rocked a similar message against the disease when she was honored with a star on the Hollywood Walk of Fame in November.
The "Anchorman" actress showed off a black and white manicure that read "FU MS" across her nails.
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Applegate previously hinted that Sunday night's SAG Awards appearance could be the final awards show of her career as she continues to fight the disease.
A close-up of Christina Applegate's SAG Awards cane with the message "FU MS." (Jordan Strauss/Invision/AP)
"It’s my last awards show as an actor probably, so it’s kind of a big deal," she told the Los Angeles Times earlier this month, referencing Sunday's SAG Awards appearance.
Applegate was nominated for the Outstanding Female Actor in a Comedy Series award for her work on "Dead to Me."
Fox News Digital's Cassie Maynard contributed to this report